Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1576540..1577195 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag FF954_RS07435 Protein ID WP_003407268.1
Coordinates 1576540..1576941 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag FF954_RS07440 Protein ID WP_003407272.1
Coordinates 1576938..1577195 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS07420 (FF954_07670) 1572012..1573397 - 1386 WP_003407260.1 cytochrome P450 -
FF954_RS07425 (FF954_07675) 1573475..1574509 + 1035 WP_003407264.1 AraC family transcriptional regulator -
FF954_RS07430 (FF954_07680) 1574555..1576285 - 1731 WP_031653809.1 PE family protein -
FF954_RS07435 (FF954_07685) 1576540..1576941 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
FF954_RS07440 (FF954_07690) 1576938..1577195 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
FF954_RS07445 (FF954_07695) 1577278..1578237 - 960 WP_003908687.1 alpha/beta hydrolase -
FF954_RS07450 (FF954_07700) 1578262..1579224 - 963 WP_003407279.1 alpha/beta hydrolase -
FF954_RS07455 (FF954_07705) 1579223..1579960 + 738 WP_031647000.1 lysoplasmalogenase -
FF954_RS07460 (FF954_07710) 1580041..1582008 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T126644 WP_003407268.1 NZ_CP040690:c1576941-1576540 [Mycobacterium tuberculosis complex sp. N0153]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T126644 NZ_CP040690:c1576941-1576540 [Mycobacterium tuberculosis complex sp. N0153]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT126644 WP_003407272.1 NZ_CP040690:c1577195-1576938 [Mycobacterium tuberculosis complex sp. N0153]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT126644 NZ_CP040690:c1577195-1576938 [Mycobacterium tuberculosis complex sp. N0153]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References