Detailed information of TA system TA126636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015927..1016546
• Replicon: chromosome
• Accession: NZ_CP040690
• Organism: Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FF954_RS04805
• Protein ID: WP_003404726.1
• Coordinates: 1016112..1016546 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: FF954_RS04800
• Protein ID: WP_003404724.1
• Coordinates: 1015927..1016106 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF954_RS04785 (FF954_04950)	1011400..1012980	+	1581	WP_003909313.1	serine hydrolase	-
FF954_RS04790 (FF954_04955)	1012977..1015370	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
FF954_RS04795	1015643..1015801	+	159	WP_003404720.1	hypothetical protein	-
FF954_RS04800 (FF954_04960)	1015927..1016106	+	180	WP_003404724.1	antitoxin	Antitoxin
FF954_RS04805 (FF954_04965)	1016112..1016546	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FF954_RS04810 (FF954_04970)	1016644..1017417	+	774	WP_031661962.1	VOC family protein	-
FF954_RS04815 (FF954_04975)	1017482..1017931	+	450	WP_003404738.1	hypothetical protein	-
FF954_RS04820 (FF954_04980)	1018066..1018296	-	231	WP_003898642.1	hypothetical protein	-
FF954_RS04825 (FF954_04985)	1018463..1019971	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FF954_RS04830 (FF954_04990)	1019973..1021212	-	1240	Protein_952	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T126636 WP_003404726.1 NZ_CP040690:1016112-1016546 [Mycobacterium tuberculosis complex sp. N0153]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T126636 NZ_CP040690:1016112-1016546 [Mycobacterium tuberculosis complex sp. N0153]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT126636 WP_003404724.1 NZ_CP040690:1015927-1016106 [Mycobacterium tuberculosis complex sp. N0153]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT126636 NZ_CP040690:1015927-1016106 [Mycobacterium tuberculosis complex sp. N0153]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5