Detailed information of TA system TA126632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 756172..756712
• Replicon: chromosome
• Accession: NZ_CP040690
• Organism: Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: FF954_RS03470
• Protein ID: WP_003403376.1
• Coordinates: 756172..756480 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FF954_RS03475
• Protein ID: WP_003403381.1
• Coordinates: 756467..756712 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF954_RS03445 (FF954_03530)	751487..752992	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FF954_RS03450 (FF954_03535)	753073..754083	+	1011	WP_031653722.1	ABC transporter ATP-binding protein	-
FF954_RS03455 (FF954_03540)	754471..754854	-	384	WP_003403365.1	ribonuclease VapC6	-
FF954_RS03460 (FF954_03545)	754949..755104	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FF954_RS03465 (FF954_03550)	755180..755896	-	717	WP_003903122.1	CPBP family intramembrane metalloprotease	-
FF954_RS03470 (FF954_03555)	756172..756480	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FF954_RS03475 (FF954_03560)	756467..756712	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FF954_RS03480 (FF954_03565)	756822..757259	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF954_RS03485 (FF954_03570)	757256..757510	-	255	WP_003911263.1	antitoxin VapB7	-
FF954_RS03490 (FF954_03575)	757624..759987	+	2364	WP_003403397.1	arylsulfatase AtsD	-
FF954_RS03495 (FF954_03580)	760050..760376	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FF954_RS03500 (FF954_03585)	760288..760626	+	339	WP_003403405.1	PIN domain-containing protein	-
FF954_RS03505 (FF954_03590)	760623..760796	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T126632 WP_003403376.1 NZ_CP040690:c756480-756172 [Mycobacterium tuberculosis complex sp. N0153]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T126632 NZ_CP040690:c756480-756172 [Mycobacterium tuberculosis complex sp. N0153]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT126632 WP_003403381.1 NZ_CP040690:c756712-756467 [Mycobacterium tuberculosis complex sp. N0153]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT126632 NZ_CP040690:c756712-756467 [Mycobacterium tuberculosis complex sp. N0153]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0