Detailed information of TA system TA126631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 754471..755104
• Replicon: chromosome
• Accession: NZ_CP040690
• Organism: Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: FF954_RS03455
• Protein ID: WP_003403365.1
• Coordinates: 754471..754854 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FF954_RS03460
• Protein ID: WP_003403368.1
• Coordinates: 754949..755104 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF954_RS03430 (FF954_03515)	749763..750299	+	537	WP_003403341.1	50S ribosomal protein L10	-
FF954_RS03435 (FF954_03520)	750336..750728	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FF954_RS03440 (FF954_03525)	750721..751416	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FF954_RS03445 (FF954_03530)	751487..752992	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FF954_RS03450 (FF954_03535)	753073..754083	+	1011	WP_031653722.1	ABC transporter ATP-binding protein	-
FF954_RS03455 (FF954_03540)	754471..754854	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
FF954_RS03460 (FF954_03545)	754949..755104	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF954_RS03465 (FF954_03550)	755180..755896	-	717	WP_003903122.1	CPBP family intramembrane metalloprotease	-
FF954_RS03470 (FF954_03555)	756172..756480	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FF954_RS03475 (FF954_03560)	756467..756712	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FF954_RS03480 (FF954_03565)	756822..757259	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF954_RS03485 (FF954_03570)	757256..757510	-	255	WP_003911263.1	antitoxin VapB7	-
FF954_RS03490 (FF954_03575)	757624..759987	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T126631 WP_003403365.1 NZ_CP040690:c754854-754471 [Mycobacterium tuberculosis complex sp. N0153]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T126631 NZ_CP040690:c754854-754471 [Mycobacterium tuberculosis complex sp. N0153]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT126631 WP_003403368.1 NZ_CP040690:c755104-754949 [Mycobacterium tuberculosis complex sp. N0153]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT126631 NZ_CP040690:c755104-754949 [Mycobacterium tuberculosis complex sp. N0153]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8