Detailed information of TA system TA126623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 641159..641835
• Replicon: chromosome
• Accession: NZ_CP040690
• Organism: Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FF954_RS02880
• Protein ID: WP_003898500.1
• Coordinates: 641159..641572 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FF954_RS02885
• Protein ID: WP_003402915.1
• Coordinates: 641569..641835 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF954_RS02850 (FF954_02905)	636504..636806	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FF954_RS02855 (FF954_02910)	636866..637144	-	279	WP_003402901.1	hypothetical protein	-
FF954_RS02860 (FF954_02915)	637141..638394	-	1254	WP_003402904.1	inorganic phosphate transporter	-
FF954_RS02865 (FF954_02920)	638514..638900	-	387	WP_003402907.1	VOC family protein	-
FF954_RS02870 (FF954_02925)	638963..639847	-	885	WP_003402909.1	SDR family oxidoreductase	-
FF954_RS02875 (FF954_02930)	639943..640845	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FF954_RS02880 (FF954_02935)	641159..641572	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF954_RS02885 (FF954_02940)	641569..641835	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
FF954_RS02890 (FF954_02945)	642027..643742	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FF954_RS02895 (FF954_02950)	643820..645424	+	1605	WP_003903056.1	amidohydrolase	-
FF954_RS02900 (FF954_02955)	645421..646401	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T126623 WP_003898500.1 NZ_CP040690:c641572-641159 [Mycobacterium tuberculosis complex sp. N0153]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T126623 NZ_CP040690:c641572-641159 [Mycobacterium tuberculosis complex sp. N0153]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT126623 WP_003402915.1 NZ_CP040690:c641835-641569 [Mycobacterium tuberculosis complex sp. N0153]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT126623 NZ_CP040690:c641835-641569 [Mycobacterium tuberculosis complex sp. N0153]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0