Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 71822..72455 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag FF954_RS00355 Protein ID WP_003400580.1
Coordinates 72054..72455 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P0CW29
Locus tag FF954_RS00350 Protein ID WP_003400577.1
Coordinates 71822..72061 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS00335 (FF954_00350) 67119..68558 + 1440 WP_003902831.1 FAD-binding oxidoreductase -
FF954_RS00340 (FF954_00355) 68614..68775 + 162 WP_003899796.1 hypothetical protein -
FF954_RS00345 (FF954_00360) 68816..71755 + 2940 Protein_65 UPF0182 family protein -
FF954_RS00350 (FF954_00365) 71822..72061 + 240 WP_003400577.1 antitoxin VapB1 Antitoxin
FF954_RS00355 (FF954_00370) 72054..72455 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
FF954_RS00360 (FF954_00375) 72507..74744 - 2238 WP_003899797.1 NADP-dependent isocitrate dehydrogenase -
FF954_RS00365 (FF954_00380) 74862..75431 - 570 WP_003902834.1 TetR/AcrR family transcriptional regulator -
FF954_RS00370 (FF954_00385) 75534..76445 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T126618 WP_003400580.1 NZ_CP040690:72054-72455 [Mycobacterium tuberculosis complex sp. N0153]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T126618 NZ_CP040690:72054-72455 [Mycobacterium tuberculosis complex sp. N0153]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 8.5960

>AT126618 WP_003400577.1 NZ_CP040690:71822-72061 [Mycobacterium tuberculosis complex sp. N0153]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT126618 NZ_CP040690:71822-72061 [Mycobacterium tuberculosis complex sp. N0153]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHM7

References