Detailed information of TA system TA126612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3190815..3191502
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: FF952_RS15170
• Protein ID: WP_003414624.1
• Coordinates: 3191059..3191502 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: FF952_RS15165
• Protein ID: WP_003414620.1
• Coordinates: 3190815..3191072 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS15145 (FF952_15665)	3186135..3186989	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FF952_RS15150 (FF952_15670)	3187045..3188208	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FF952_RS15155 (FF952_15675)	3188225..3189439	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FF952_RS15160 (FF952_15680)	3189447..3190688	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FF952_RS15165 (FF952_15685)	3190815..3191072	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
FF952_RS15170 (FF952_15690)	3191059..3191502	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF952_RS15175 (FF952_15695)	3191582..3192244	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
FF952_RS15180 (FF952_15700)	3192340..3192531	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
FF952_RS15185 (FF952_15705)	3192863..3194611	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
FF952_RS15190 (FF952_15710)	3194707..3195288	+	582	WP_003414644.1	fasciclin domain-containing protein	-
FF952_RS15195 (FF952_15715)	3195388..3195654	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T126612 WP_003414624.1 NZ_CP040689:3191059-3191502 [Mycobacterium tuberculosis complex sp. N0072]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T126612 NZ_CP040689:3191059-3191502 [Mycobacterium tuberculosis complex sp. N0072]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT126612 WP_003414620.1 NZ_CP040689:3190815-3191072 [Mycobacterium tuberculosis complex sp. N0072]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT126612 NZ_CP040689:3190815-3191072 [Mycobacterium tuberculosis complex sp. N0072]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0