Detailed information of TA system TA126601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2871837..2872477
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FF952_RS13445
• Protein ID: WP_003412970.1
• Coordinates: 2871837..2872256 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FF952_RS13450
• Protein ID: WP_003412975.1
• Coordinates: 2872253..2872477 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS13415 (FF952_13885)	2867422..2868144	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FF952_RS13420 (FF952_13895)	2868661..2868888	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
FF952_RS13425 (FF952_13900)	2868885..2869286	+	402	WP_003412963.1	PIN domain-containing protein	-
FF952_RS13430 (FF952_13905)	2869321..2870241	-	921	WP_003412965.1	restriction endonuclease	-
FF952_RS13435	2870582..2870827	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FF952_RS13440 (FF952_13910)	2870886..2871836	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
FF952_RS13445 (FF952_13915)	2871837..2872256	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF952_RS13450 (FF952_13920)	2872253..2872477	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FF952_RS13455 (FF952_13925)	2872509..2875352	-	2844	WP_003904863.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FF952_RS13460 (FF952_13930)	2875424..2875825	-	402	WP_003412981.1	hypothetical protein	-
FF952_RS13465 (FF952_13935)	2875825..2876295	-	471	WP_003412985.1	transcription antitermination factor NusB	-
FF952_RS13470 (FF952_13940)	2876298..2876861	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T126601 WP_003412970.1 NZ_CP040689:c2872256-2871837 [Mycobacterium tuberculosis complex sp. N0072]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T126601 NZ_CP040689:c2872256-2871837 [Mycobacterium tuberculosis complex sp. N0072]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT126601 WP_003412975.1 NZ_CP040689:c2872477-2872253 [Mycobacterium tuberculosis complex sp. N0072]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT126601 NZ_CP040689:c2872477-2872253 [Mycobacterium tuberculosis complex sp. N0072]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09