Detailed information of TA system TA126597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2343070..2343706
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FF952_RS10950
• Protein ID: WP_003904769.1
• Coordinates: 2343296..2343706 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: FF952_RS10945
• Protein ID: WP_003410651.1
• Coordinates: 2343070..2343303 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS10930	2338527..2338919	+	393	WP_229303073.1	metal ABC transporter permease	-
FF952_RS10935 (FF952_11310)	2338920..2339324	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
FF952_RS10940 (FF952_11315)	2339408..2342992	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
FF952_RS10945 (FF952_11320)	2343070..2343303	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
FF952_RS10950 (FF952_11325)	2343296..2343706	+	411	WP_003904769.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
FF952_RS10955 (FF952_11330)	2343690..2344781	+	1092	WP_003900454.1	precorrin-3B synthase	-
FF952_RS10960 (FF952_11335)	2344791..2345417	+	627	WP_031680792.1	precorrin-8X methylmutase	-
FF952_RS10965 (FF952_11340)	2345414..2346940	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
FF952_RS10970 (FF952_11345)	2346886..2348109	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14176.35 Da
• Isoelectric Point: 9.4835

>T126597 WP_003904769.1 NZ_CP040689:2343296-2343706 [Mycobacterium tuberculosis complex sp. N0072]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMPAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T126597 NZ_CP040689:2343296-2343706 [Mycobacterium tuberculosis complex sp. N0072]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCCCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT126597 WP_003410651.1 NZ_CP040689:2343070-2343303 [Mycobacterium tuberculosis complex sp. N0072]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT126597 NZ_CP040689:2343070-2343303 [Mycobacterium tuberculosis complex sp. N0072]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50