Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1579641..1580296 Replicon chromosome
Accession NZ_CP040689
Organism Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag FF952_RS07445 Protein ID WP_003407268.1
Coordinates 1579641..1580042 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag FF952_RS07450 Protein ID WP_003407272.1
Coordinates 1580039..1580296 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF952_RS07430 (FF952_07665) 1575113..1576498 - 1386 WP_003407260.1 cytochrome P450 -
FF952_RS07435 (FF952_07670) 1576576..1577610 + 1035 WP_003407264.1 AraC family transcriptional regulator -
FF952_RS07440 (FF952_07675) 1577656..1579386 - 1731 WP_031653809.1 PE family protein -
FF952_RS07445 (FF952_07680) 1579641..1580042 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
FF952_RS07450 (FF952_07685) 1580039..1580296 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
FF952_RS07455 (FF952_07690) 1580379..1581338 - 960 WP_015631299.1 alpha/beta hydrolase -
FF952_RS07460 (FF952_07695) 1581363..1582325 - 963 WP_003407279.1 alpha/beta hydrolase -
FF952_RS07465 (FF952_07700) 1582324..1583061 + 738 WP_031647000.1 lysoplasmalogenase -
FF952_RS07470 (FF952_07705) 1583142..1585109 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T126584 WP_003407268.1 NZ_CP040689:c1580042-1579641 [Mycobacterium tuberculosis complex sp. N0072]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T126584 NZ_CP040689:c1580042-1579641 [Mycobacterium tuberculosis complex sp. N0072]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT126584 WP_003407272.1 NZ_CP040689:c1580296-1580039 [Mycobacterium tuberculosis complex sp. N0072]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT126584 NZ_CP040689:c1580296-1580039 [Mycobacterium tuberculosis complex sp. N0072]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References