Detailed information of TA system TA126576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1018797..1019416
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FF952_RS04815
• Protein ID: WP_003404726.1
• Coordinates: 1018982..1019416 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: FF952_RS04810
• Protein ID: WP_003404724.1
• Coordinates: 1018797..1018976 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS04795 (FF952_04950)	1014270..1015850	+	1581	WP_003909313.1	serine hydrolase	-
FF952_RS04800 (FF952_04955)	1015847..1018240	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
FF952_RS04805	1018513..1018671	+	159	WP_003404720.1	hypothetical protein	-
FF952_RS04810 (FF952_04960)	1018797..1018976	+	180	WP_003404724.1	antitoxin	Antitoxin
FF952_RS04815 (FF952_04965)	1018982..1019416	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FF952_RS04820 (FF952_04970)	1019514..1020287	+	774	WP_003404735.1	VOC family protein	-
FF952_RS04825 (FF952_04975)	1020352..1020801	+	450	WP_003404738.1	hypothetical protein	-
FF952_RS04830 (FF952_04980)	1020936..1021166	-	231	WP_031656773.1	hypothetical protein	-
FF952_RS04835 (FF952_04985)	1021333..1022841	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FF952_RS04840 (FF952_04990)	1022843..1024081	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T126576 WP_003404726.1 NZ_CP040689:1018982-1019416 [Mycobacterium tuberculosis complex sp. N0072]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T126576 NZ_CP040689:1018982-1019416 [Mycobacterium tuberculosis complex sp. N0072]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT126576 WP_003404724.1 NZ_CP040689:1018797-1018976 [Mycobacterium tuberculosis complex sp. N0072]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT126576 NZ_CP040689:1018797-1018976 [Mycobacterium tuberculosis complex sp. N0072]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5