Detailed information of TA system TA126572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 759591..760131
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: FF952_RS03485
• Protein ID: WP_003403376.1
• Coordinates: 759591..759899 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FF952_RS03490
• Protein ID: WP_003403381.1
• Coordinates: 759886..760131 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS03460 (FF952_03540)	754906..756411	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FF952_RS03465 (FF952_03545)	756492..757502	+	1011	WP_031653722.1	ABC transporter ATP-binding protein	-
FF952_RS03470 (FF952_03550)	757890..758273	-	384	WP_003403365.1	ribonuclease VapC6	-
FF952_RS03475 (FF952_03555)	758368..758523	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FF952_RS03480 (FF952_03560)	758599..759315	-	717	WP_003903122.1	CPBP family intramembrane metalloprotease	-
FF952_RS03485 (FF952_03565)	759591..759899	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FF952_RS03490 (FF952_03570)	759886..760131	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FF952_RS03495 (FF952_03575)	760241..760678	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF952_RS03500 (FF952_03580)	760675..760929	-	255	WP_003911263.1	antitoxin VapB7	-
FF952_RS03505 (FF952_03585)	761043..763406	+	2364	WP_003403397.1	arylsulfatase AtsD	-
FF952_RS03510 (FF952_03590)	763469..763795	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FF952_RS03515 (FF952_03595)	763707..764045	+	339	WP_003403405.1	PIN domain-containing protein	-
FF952_RS03520 (FF952_03600)	764042..764215	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T126572 WP_003403376.1 NZ_CP040689:c759899-759591 [Mycobacterium tuberculosis complex sp. N0072]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T126572 NZ_CP040689:c759899-759591 [Mycobacterium tuberculosis complex sp. N0072]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT126572 WP_003403381.1 NZ_CP040689:c760131-759886 [Mycobacterium tuberculosis complex sp. N0072]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT126572 NZ_CP040689:c760131-759886 [Mycobacterium tuberculosis complex sp. N0072]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0