Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 759591..760131 Replicon chromosome
Accession NZ_CP040689
Organism Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag FF952_RS03485 Protein ID WP_003403376.1
Coordinates 759591..759899 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag FF952_RS03490 Protein ID WP_003403381.1
Coordinates 759886..760131 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF952_RS03460 (FF952_03540) 754906..756411 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
FF952_RS03465 (FF952_03545) 756492..757502 + 1011 WP_031653722.1 ABC transporter ATP-binding protein -
FF952_RS03470 (FF952_03550) 757890..758273 - 384 WP_003403365.1 ribonuclease VapC6 -
FF952_RS03475 (FF952_03555) 758368..758523 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
FF952_RS03480 (FF952_03560) 758599..759315 - 717 WP_003903122.1 CPBP family intramembrane metalloprotease -
FF952_RS03485 (FF952_03565) 759591..759899 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FF952_RS03490 (FF952_03570) 759886..760131 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
FF952_RS03495 (FF952_03575) 760241..760678 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
FF952_RS03500 (FF952_03580) 760675..760929 - 255 WP_003911263.1 antitoxin VapB7 -
FF952_RS03505 (FF952_03585) 761043..763406 + 2364 WP_003403397.1 arylsulfatase AtsD -
FF952_RS03510 (FF952_03590) 763469..763795 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF952_RS03515 (FF952_03595) 763707..764045 + 339 WP_003403405.1 PIN domain-containing protein -
FF952_RS03520 (FF952_03600) 764042..764215 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T126572 WP_003403376.1 NZ_CP040689:c759899-759591 [Mycobacterium tuberculosis complex sp. N0072]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T126572 NZ_CP040689:c759899-759591 [Mycobacterium tuberculosis complex sp. N0072]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT126572 WP_003403381.1 NZ_CP040689:c760131-759886 [Mycobacterium tuberculosis complex sp. N0072]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT126572 NZ_CP040689:c760131-759886 [Mycobacterium tuberculosis complex sp. N0072]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References