Detailed information of TA system TA126571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 757890..758523
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: FF952_RS03470
• Protein ID: WP_003403365.1
• Coordinates: 757890..758273 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FF952_RS03475
• Protein ID: WP_003403368.1
• Coordinates: 758368..758523 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS03445 (FF952_03525)	753182..753718	+	537	WP_003403341.1	50S ribosomal protein L10	-
FF952_RS03450 (FF952_03530)	753755..754147	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FF952_RS03455 (FF952_03535)	754140..754835	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FF952_RS03460 (FF952_03540)	754906..756411	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FF952_RS03465 (FF952_03545)	756492..757502	+	1011	WP_031653722.1	ABC transporter ATP-binding protein	-
FF952_RS03470 (FF952_03550)	757890..758273	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
FF952_RS03475 (FF952_03555)	758368..758523	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF952_RS03480 (FF952_03560)	758599..759315	-	717	WP_003903122.1	CPBP family intramembrane metalloprotease	-
FF952_RS03485 (FF952_03565)	759591..759899	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FF952_RS03490 (FF952_03570)	759886..760131	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FF952_RS03495 (FF952_03575)	760241..760678	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF952_RS03500 (FF952_03580)	760675..760929	-	255	WP_003911263.1	antitoxin VapB7	-
FF952_RS03505 (FF952_03585)	761043..763406	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T126571 WP_003403365.1 NZ_CP040689:c758273-757890 [Mycobacterium tuberculosis complex sp. N0072]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T126571 NZ_CP040689:c758273-757890 [Mycobacterium tuberculosis complex sp. N0072]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT126571 WP_003403368.1 NZ_CP040689:c758523-758368 [Mycobacterium tuberculosis complex sp. N0072]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT126571 NZ_CP040689:c758523-758368 [Mycobacterium tuberculosis complex sp. N0072]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8