Detailed information of TA system TA126570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 722404..723068
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P96917
• Locus tag: FF952_RS03300
• Protein ID: WP_003403246.1
• Coordinates: 722661..723068 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WF18
• Locus tag: FF952_RS03295
• Protein ID: WP_003403244.1
• Coordinates: 722404..722664 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS03270 (FF952_03350)	718581..719738	+	1158	WP_003903091.1	hypothetical protein	-
FF952_RS03275 (FF952_03355)	719749..720696	+	948	WP_003403232.1	DUF732 domain-containing protein	-
FF952_RS03280 (FF952_03360)	720789..721043	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	-
FF952_RS03285 (FF952_03365)	721043..721438	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	-
FF952_RS03290 (FF952_03370)	721532..722272	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
FF952_RS03295 (FF952_03375)	722404..722664	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FF952_RS03300 (FF952_03380)	722661..723068	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF952_RS03305 (FF952_03385)	723140..724291	-	1152	WP_244736125.1	FIST N-terminal domain-containing protein	-
FF952_RS03310 (FF952_03390)	724384..726105	-	1722	WP_286450986.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-57)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14376.37 Da
• Isoelectric Point: 4.3568

>T126570 WP_003403246.1 NZ_CP040689:722661-723068 [Mycobacterium tuberculosis complex sp. N0072]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Nucleotide

• Length: 408 bp

>T126570 NZ_CP040689:722661-723068 [Mycobacterium tuberculosis complex sp. N0072]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9492.84 Da
• Isoelectric Point: 11.9108

>AT126570 WP_003403244.1 NZ_CP040689:722404-722664 [Mycobacterium tuberculosis complex sp. N0072]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Nucleotide

• Length: 261 bp

>AT126570 NZ_CP040689:722404-722664 [Mycobacterium tuberculosis complex sp. N0072]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• PDB: 3DBO

Antitoxin

• AlphaFold DB: A0A7U4BSE4