Detailed information of TA system TA126565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 699217..699863
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: FF952_RS03130
• Protein ID: WP_003403122.1
• Coordinates: 699217..699609 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: FF952_RS03135
• Protein ID: WP_003403125.1
• Coordinates: 699606..699863 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS03115 (FF952_03180)	694879..696405	+	1527	WP_003403112.1	virulence factor Mce family protein	-
FF952_RS03120 (FF952_03185)	696468..697610	+	1143	WP_003911253.1	virulence factor Mce family protein	-
FF952_RS03125 (FF952_03190)	697615..699165	+	1551	WP_003403119.1	MlaD family protein	-
FF952_RS03130 (FF952_03195)	699217..699609	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
FF952_RS03135 (FF952_03200)	699606..699863	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
FF952_RS03140 (FF952_03205)	700046..701281	-	1236	WP_003403128.1	ATP-binding protein	-
FF952_RS03145 (FF952_03210)	701532..701945	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
FF952_RS03150 (FF952_03215)	701942..702178	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	-
FF952_RS03155 (FF952_03220)	702282..702788	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FF952_RS03160 (FF952_03225)	702902..703432	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FF952_RS03165 (FF952_03230)	703416..704111	-	696	WP_200861092.1	two-component system response regulator TcrA	-
FF952_RS03170 (FF952_03235)	704234..704545	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T126565 WP_003403122.1 NZ_CP040689:c699609-699217 [Mycobacterium tuberculosis complex sp. N0072]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T126565 NZ_CP040689:c699609-699217 [Mycobacterium tuberculosis complex sp. N0072]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT126565 WP_003403125.1 NZ_CP040689:c699863-699606 [Mycobacterium tuberculosis complex sp. N0072]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT126565 NZ_CP040689:c699863-699606 [Mycobacterium tuberculosis complex sp. N0072]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86