Detailed information of TA system TA126563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 644578..645254
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FF952_RS02895
• Protein ID: WP_003898500.1
• Coordinates: 644578..644991 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FF952_RS02900
• Protein ID: WP_003402915.1
• Coordinates: 644988..645254 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS02865 (FF952_02915)	639923..640225	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FF952_RS02870 (FF952_02920)	640285..640563	-	279	WP_003402901.1	hypothetical protein	-
FF952_RS02875 (FF952_02925)	640560..641813	-	1254	WP_244736122.1	inorganic phosphate transporter	-
FF952_RS02880 (FF952_02930)	641933..642319	-	387	WP_003402907.1	VOC family protein	-
FF952_RS02885 (FF952_02935)	642382..643266	-	885	WP_003402909.1	SDR family oxidoreductase	-
FF952_RS02890 (FF952_02940)	643362..644264	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FF952_RS02895 (FF952_02945)	644578..644991	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF952_RS02900 (FF952_02950)	644988..645254	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
FF952_RS02905 (FF952_02955)	645446..647161	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FF952_RS02910 (FF952_02960)	647239..648843	+	1605	WP_003903056.1	amidohydrolase	-
FF952_RS02915 (FF952_02965)	648840..649820	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T126563 WP_003898500.1 NZ_CP040689:c644991-644578 [Mycobacterium tuberculosis complex sp. N0072]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T126563 NZ_CP040689:c644991-644578 [Mycobacterium tuberculosis complex sp. N0072]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT126563 WP_003402915.1 NZ_CP040689:c645254-644988 [Mycobacterium tuberculosis complex sp. N0072]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT126563 NZ_CP040689:c645254-644988 [Mycobacterium tuberculosis complex sp. N0072]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0