Detailed information of TA system TA126560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 367083..367654
• Replicon: chromosome
• Accession: NZ_CP040689
• Organism: Mycobacterium tuberculosis complex sp. N0072

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FF952_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 367083..367385 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FF952_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 367433..367654 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF952_RS01575 (FF952_01605)	362561..363364	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
FF952_RS01580 (FF952_01610)	363374..364771	-	1398	WP_003401544.1	sulfatase	-
FF952_RS01585 (FF952_01615)	364950..366716	+	1767	WP_286450958.1	PE family protein	-
FF952_RS01590 (FF952_01620)	366859..367086	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FF952_RS01595 (FF952_01625)	367083..367385	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FF952_RS01600 (FF952_01630)	367433..367654	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FF952_RS01605 (FF952_01635)	367651..368076	+	426	WP_003401566.1	PIN domain nuclease	-
FF952_RS01610 (FF952_01640)	368212..368844	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
FF952_RS01615 (FF952_01645)	368841..369749	+	909	WP_003900117.1	protochlorophyllide reductase	-
FF952_RS01620	369757..370347	-	591	WP_229298012.1	hypothetical protein	-
FF952_RS01625	370340..370390	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T126560 WP_003401560.1 NZ_CP040689:367083-367385 [Mycobacterium tuberculosis complex sp. N0072]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T126560 NZ_CP040689:367083-367385 [Mycobacterium tuberculosis complex sp. N0072]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT126560 WP_003401563.1 NZ_CP040689:367433-367654 [Mycobacterium tuberculosis complex sp. N0072]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT126560 NZ_CP040689:367433-367654 [Mycobacterium tuberculosis complex sp. N0072]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48