Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3097085..3097655 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FF951_RS14775 Protein ID WP_003414166.1
Coordinates 3097085..3097441 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FF951_RS14780 Protein ID WP_003901465.1
Coordinates 3097425..3097655 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS14755 (FF951_15245) 3092537..3094225 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FF951_RS14760 (FF951_15250) 3094229..3094555 - 327 WP_003414157.1 hypothetical protein -
FF951_RS14765 (FF951_15255) 3094728..3095315 + 588 WP_003914429.1 DUF3558 family protein -
FF951_RS14770 (FF951_15260) 3095334..3096983 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FF951_RS14775 (FF951_15265) 3097085..3097441 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FF951_RS14780 (FF951_15270) 3097425..3097655 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
FF951_RS14785 (FF951_15275) 3097698..3098741 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FF951_RS14790 3098740..3099207 + 468 WP_003414177.1 DUF1778 domain-containing protein -
FF951_RS14795 3099383..3099637 - 255 WP_003917684.1 hypothetical protein -
FF951_RS14800 (FF951_15290) 3099785..3100189 + 405 WP_003414181.1 hypothetical protein -
FF951_RS14805 (FF951_15295) 3100186..3100377 + 192 WP_003414184.1 hypothetical protein -
FF951_RS14810 3100594..3100854 + 261 Protein_2923 transposase -
FF951_RS14815 (FF951_15305) 3101964..3102221 + 258 WP_003899489.1 hypothetical protein -
FF951_RS14820 (FF951_15310) 3102326..3102637 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-113)

Antitoxin

(3-29)


Sequences


Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T126548 WP_003414166.1 NZ_CP040688:c3097441-3097085 [Mycobacterium tuberculosis complex sp. N0052]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T126548 NZ_CP040688:c3097441-3097085 [Mycobacterium tuberculosis complex sp. N0052]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT126548 WP_003901465.1 NZ_CP040688:c3097655-3097425 [Mycobacterium tuberculosis complex sp. N0052]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT126548 NZ_CP040688:c3097655-3097425 [Mycobacterium tuberculosis complex sp. N0052]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS


Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References