Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2857745..2858382 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FF951_RS13460 Protein ID WP_003413180.1
Coordinates 2857745..2858140 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FF951_RS13465 Protein ID WP_003413183.1
Coordinates 2858137..2858382 (-) Length 82 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS13420 (FF951_13860) 2853149..2854360 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
FF951_RS13425 (FF951_13870) 2854487..2855146 + 660 WP_003902296.1 LppA family lipoprotein -
FF951_RS13430 (FF951_13875) 2855143..2855805 + 663 WP_003905878.1 LppA family lipoprotein -
FF951_RS13435 (FF951_13880) 2855802..2856079 + 278 Protein_2652 type II toxin-antitoxin system VapB family antitoxin -
FF951_RS13440 (FF951_13885) 2856172..2856585 + 414 WP_003413164.1 PIN domain nuclease -
FF951_RS13445 (FF951_13890) 2856624..2856881 + 258 WP_003413167.1 CopG family transcriptional regulator -
FF951_RS13450 (FF951_13895) 2856878..2857255 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FF951_RS13455 (FF951_13900) 2857271..2857645 - 375 WP_003413177.1 hypothetical protein -
FF951_RS13460 (FF951_13905) 2857745..2858140 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FF951_RS13465 (FF951_13910) 2858137..2858382 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FF951_RS13470 (FF951_13920) 2858793..2859212 - 420 WP_003413190.1 A24 family peptidase -
FF951_RS13475 (FF951_13925) 2859224..2860033 - 810 WP_003413193.1 shikimate dehydrogenase -
FF951_RS13480 (FF951_13930) 2860030..2861283 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FF951_RS13485 (FF951_13935) 2861276..2861788 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-129)

Antitoxin

(11-49)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T126543 WP_003413180.1 NZ_CP040688:c2858140-2857745 [Mycobacterium tuberculosis complex sp. N0052]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T126543 NZ_CP040688:c2858140-2857745 [Mycobacterium tuberculosis complex sp. N0052]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT126543 WP_003413183.1 NZ_CP040688:c2858382-2858137 [Mycobacterium tuberculosis complex sp. N0052]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT126543 NZ_CP040688:c2858382-2858137 [Mycobacterium tuberculosis complex sp. N0052]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF


Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References