Detailed information of TA system TA126540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2842286..2842926
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FF951_RS13355
• Protein ID: WP_003412970.1
• Coordinates: 2842286..2842705 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FF951_RS13360
• Protein ID: WP_003412975.1
• Coordinates: 2842702..2842926 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS13325 (FF951_13765)	2837876..2838598	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FF951_RS13330 (FF951_13775)	2839115..2839337	+	223	Protein_2631	type II toxin-antitoxin system VapB family antitoxin	-
FF951_RS13335 (FF951_13780)	2839334..2839735	+	402	WP_003412963.1	PIN domain-containing protein	-
FF951_RS13340 (FF951_13785)	2839770..2840690	-	921	WP_003902293.1	restriction endonuclease	-
FF951_RS13345	2841031..2841276	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FF951_RS13350 (FF951_13790)	2841335..2842285	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
FF951_RS13355 (FF951_13795)	2842286..2842705	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF951_RS13360 (FF951_13800)	2842702..2842926	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FF951_RS13365 (FF951_13805)	2842957..2845800	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FF951_RS13370 (FF951_13810)	2845872..2846273	-	402	WP_003412981.1	hypothetical protein	-
FF951_RS13375 (FF951_13815)	2846273..2846743	-	471	WP_003899364.1	transcription antitermination factor NusB	-
FF951_RS13380 (FF951_13820)	2846746..2847309	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T126540 WP_003412970.1 NZ_CP040688:c2842705-2842286 [Mycobacterium tuberculosis complex sp. N0052]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T126540 NZ_CP040688:c2842705-2842286 [Mycobacterium tuberculosis complex sp. N0052]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT126540 WP_003412975.1 NZ_CP040688:c2842926-2842702 [Mycobacterium tuberculosis complex sp. N0052]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT126540 NZ_CP040688:c2842926-2842702 [Mycobacterium tuberculosis complex sp. N0052]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09