Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2312945..2313581 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag FF951_RS10835 Protein ID WP_003410654.1
Coordinates 2313171..2313581 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag FF951_RS10830 Protein ID WP_003410651.1
Coordinates 2312945..2313178 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS10815 2308393..2308794 + 402 WP_009937711.1 metal ABC transporter permease -
FF951_RS10820 (FF951_11180) 2308795..2309199 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
FF951_RS10825 (FF951_11185) 2309283..2312867 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
FF951_RS10830 (FF951_11190) 2312945..2313178 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
FF951_RS10835 (FF951_11195) 2313171..2313581 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
FF951_RS10840 (FF951_11200) 2313565..2314656 + 1092 WP_003900454.1 precorrin-3B synthase -
FF951_RS10845 (FF951_11205) 2314666..2315292 + 627 WP_003410658.1 precorrin-8X methylmutase -
FF951_RS10850 (FF951_11210) 2315289..2316815 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
FF951_RS10855 (FF951_11215) 2316761..2317984 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T126536 WP_003410654.1 NZ_CP040688:2313171-2313581 [Mycobacterium tuberculosis complex sp. N0052]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T126536 NZ_CP040688:2313171-2313581 [Mycobacterium tuberculosis complex sp. N0052]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT126536 WP_003410651.1 NZ_CP040688:2312945-2313178 [Mycobacterium tuberculosis complex sp. N0052]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT126536 NZ_CP040688:2312945-2313178 [Mycobacterium tuberculosis complex sp. N0052]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References