126533

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2218706..2219292 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FF951_RS10420 Protein ID WP_003410010.1
Coordinates 2218706..2219050 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FF951_RS10425 Protein ID WP_003410014.1
Coordinates 2219044..2219292 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS10385 (FF951_10730) 2214412..2215011 + 600 WP_286449251.1 L-lysine exporter -
FF951_RS10390 (FF951_10735) 2215427..2215855 + 429 WP_003409992.1 cellulose-binding protein -
FF951_RS10395 (FF951_10740) 2216081..2216620 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FF951_RS10400 (FF951_10750) 2217140..2217700 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
FF951_RS10405 (FF951_10755) 2217697..2218038 - 342 WP_003410003.1 DUF2384 domain-containing protein -
FF951_RS10410 (FF951_10760) 2218124..2218381 + 258 WP_003410006.1 hypothetical protein -
FF951_RS10415 (FF951_10765) 2218282..2218617 - 336 WP_003410009.1 dehydrogenase -
FF951_RS10420 (FF951_10770) 2218706..2219050 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FF951_RS10425 (FF951_10775) 2219044..2219292 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FF951_RS10430 (FF951_10780) 2219392..2221707 - 2316 WP_003899120.1 cation transporter ATPase CptG -
FF951_RS10435 (FF951_10785) 2221704..2221976 - 273 WP_003410017.1 DUF1490 family protein -
FF951_RS10440 (FF951_10790) 2222029..2222385 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FF951_RS10445 (FF951_10795) 2222542..2223309 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T126533 WP_003410010.1 NZ_CP040688:c2219050-2218706 [Mycobacterium tuberculosis complex sp. N0052]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T126533 NZ_CP040688:c2219050-2218706 [Mycobacterium tuberculosis complex sp. N0052]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT126533 WP_003410014.1 NZ_CP040688:c2219292-2219044 [Mycobacterium tuberculosis complex sp. N0052]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT126533 NZ_CP040688:c2219292-2219044 [Mycobacterium tuberculosis complex sp. N0052]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References