Detailed information of TA system TA126531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2209814..2210502
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: FF951_RS10355
• Protein ID: WP_003409958.1
• Coordinates: 2209814..2210233 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: FF951_RS10360
• Protein ID: WP_003409968.1
• Coordinates: 2210242..2210502 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS10335 (FF951_10680)	2205309..2206157	+	849	WP_003901306.1	class I SAM-dependent methyltransferase	-
FF951_RS10340 (FF951_10685)	2206120..2207565	-	1446	WP_003899115.1	APC family permease	-
FF951_RS10345 (FF951_10690)	2207744..2208430	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
FF951_RS10350 (FF951_10695)	2208621..2209589	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
FF951_RS10355 (FF951_10700)	2209814..2210233	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF951_RS10360 (FF951_10705)	2210242..2210502	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF951_RS10365 (FF951_10710)	2210645..2212321	+	1677	WP_003899118.1	PecA family PE domain-processing aspartic protease	-
FF951_RS10370 (FF951_10715)	2212309..2212962	-	654	WP_003409976.1	cutinase Cfp21	-
FF951_RS10375 (FF951_10720)	2213077..2213307	+	231	WP_003409980.1	DUF167 domain-containing protein	-
FF951_RS10380 (FF951_10725)	2213392..2214303	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
FF951_RS10385 (FF951_10730)	2214412..2215011	+	600	WP_286449251.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T126531 WP_003409958.1 NZ_CP040688:c2210233-2209814 [Mycobacterium tuberculosis complex sp. N0052]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T126531 NZ_CP040688:c2210233-2209814 [Mycobacterium tuberculosis complex sp. N0052]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT126531 WP_003409968.1 NZ_CP040688:c2210502-2210242 [Mycobacterium tuberculosis complex sp. N0052]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT126531 NZ_CP040688:c2210502-2210242 [Mycobacterium tuberculosis complex sp. N0052]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483