Detailed information of TA system TA126525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1762749..1763362
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: FF951_RS08265
• Protein ID: WP_003407786.1
• Coordinates: 1762958..1763362 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: FF951_RS08260
• Protein ID: WP_009935474.1
• Coordinates: 1762749..1762952 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS08230 (FF951_08495)	1758154..1758534	+	381	WP_003407768.1	fumarate reductase subunit C	-
FF951_RS08235 (FF951_08500)	1758531..1758908	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
FF951_RS08240 (FF951_08505)	1758976..1759584	+	609	WP_003902101.1	TetR/AcrR family transcriptional regulator	-
FF951_RS08245 (FF951_08510)	1759768..1760916	+	1149	Protein_1626	MMPL family transporter	-
FF951_RS08250 (FF951_08515)	1760926..1761372	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
FF951_RS08255 (FF951_08520)	1761407..1762696	+	1290	WP_003407781.1	threonine ammonia-lyase	-
FF951_RS08260 (FF951_08525)	1762749..1762952	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF951_RS08265 (FF951_08530)	1762958..1763362	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
FF951_RS08270 (FF951_08535)	1763379..1765121	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
FF951_RS08275 (FF951_08540)	1765114..1767411	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T126525 WP_003407786.1 NZ_CP040688:1762958-1763362 [Mycobacterium tuberculosis complex sp. N0052]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T126525 NZ_CP040688:1762958-1763362 [Mycobacterium tuberculosis complex sp. N0052]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT126525 WP_009935474.1 NZ_CP040688:1762749-1762952 [Mycobacterium tuberculosis complex sp. N0052]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT126525 NZ_CP040688:1762749-1762952 [Mycobacterium tuberculosis complex sp. N0052]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46