Detailed information of TA system TA126519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1234201..1234769
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FF951_RS05910
• Protein ID: WP_003405865.1
• Coordinates: 1234395..1234769 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FF951_RS05905
• Protein ID: WP_003405863.1
• Coordinates: 1234201..1234398 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS05885 (FF951_06055)	1230242..1230880	-	639	WP_003898730.1	lipid droplet-associated protein	-
FF951_RS05890 (FF951_06060)	1230970..1231977	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FF951_RS05895 (FF951_06065)	1231994..1232977	-	984	WP_003898731.1	hypothetical protein	-
FF951_RS05900 (FF951_06070)	1233040..1234113	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FF951_RS05905 (FF951_06075)	1234201..1234398	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF951_RS05910 (FF951_06080)	1234395..1234769	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF951_RS05915 (FF951_06085)	1234972..1235670	+	699	WP_003898733.1	hypothetical protein	-
FF951_RS05920 (FF951_06090)	1235788..1235973	+	186	WP_003901093.1	hypothetical protein	-
FF951_RS05925 (FF951_06095)	1235900..1236175	-	276	WP_003405867.1	hypothetical protein	-
FF951_RS05930 (FF951_06100)	1236418..1236741	+	324	WP_003405871.1	putative quinol monooxygenase	-
FF951_RS05935 (FF951_06105)	1236756..1237454	-	699	WP_031646953.1	hypothetical protein	-
FF951_RS05940	1237488..1237697	+	210	WP_003911400.1	hypothetical protein	-
FF951_RS05945 (FF951_06110)	1237649..1238419	-	771	Protein_1171	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T126519 WP_003405865.1 NZ_CP040688:1234395-1234769 [Mycobacterium tuberculosis complex sp. N0052]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T126519 NZ_CP040688:1234395-1234769 [Mycobacterium tuberculosis complex sp. N0052]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT126519 WP_003405863.1 NZ_CP040688:1234201-1234398 [Mycobacterium tuberculosis complex sp. N0052]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT126519 NZ_CP040688:1234201-1234398 [Mycobacterium tuberculosis complex sp. N0052]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07