Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1225445..1226076 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag FF951_RS05855 Protein ID WP_003405820.1
Coordinates 1225445..1225756 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag FF951_RS05860 Protein ID WP_003405836.1
Coordinates 1225756..1226076 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS05835 (FF951_06005) 1220926..1222350 - 1425 WP_003405805.1 class II fumarate hydratase -
FF951_RS05840 (FF951_06010) 1222381..1223469 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
FF951_RS05845 (FF951_06015) 1223525..1224169 + 645 WP_003915494.1 DUF4245 domain-containing protein -
FF951_RS05850 (FF951_06020) 1224176..1225333 - 1158 WP_003898727.1 AI-2E family transporter -
FF951_RS05855 (FF951_06025) 1225445..1225756 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
FF951_RS05860 (FF951_06030) 1225756..1226076 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
FF951_RS05865 (FF951_06035) 1226086..1227622 + 1537 Protein_1155 carboxylesterase/lipase family protein -
FF951_RS05870 (FF951_06040) 1227629..1228741 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
FF951_RS05875 (FF951_06045) 1228751..1229008 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
FF951_RS05880 (FF951_06050) 1228998..1230245 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
FF951_RS05885 (FF951_06055) 1230242..1230880 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T126518 WP_003405820.1 NZ_CP040688:c1225756-1225445 [Mycobacterium tuberculosis complex sp. N0052]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T126518 NZ_CP040688:c1225756-1225445 [Mycobacterium tuberculosis complex sp. N0052]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT126518 WP_003405836.1 NZ_CP040688:c1226076-1225756 [Mycobacterium tuberculosis complex sp. N0052]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT126518 NZ_CP040688:c1226076-1225756 [Mycobacterium tuberculosis complex sp. N0052]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References