Detailed information of TA system TA126515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1009485..1010104
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FF951_RS04780
• Protein ID: WP_003404726.1
• Coordinates: 1009670..1010104 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: FF951_RS04775
• Protein ID: WP_003898641.1
• Coordinates: 1009485..1009664 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS04760 (FF951_04910)	1004958..1006538	+	1581	WP_003909313.1	serine hydrolase	-
FF951_RS04765 (FF951_04915)	1006535..1008928	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
FF951_RS04770	1009201..1009359	+	159	WP_003404720.1	hypothetical protein	-
FF951_RS04775 (FF951_04920)	1009485..1009664	+	180	WP_003898641.1	antitoxin	Antitoxin
FF951_RS04780 (FF951_04925)	1009670..1010104	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FF951_RS04785 (FF951_04930)	1010202..1010975	+	774	WP_003404735.1	VOC family protein	-
FF951_RS04790 (FF951_04935)	1011040..1011489	+	450	WP_003404738.1	hypothetical protein	-
FF951_RS04795 (FF951_04940)	1011624..1011854	-	231	WP_003898642.1	hypothetical protein	-
FF951_RS04800 (FF951_04945)	1012021..1013529	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FF951_RS04805 (FF951_04950)	1013531..1014769	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T126515 WP_003404726.1 NZ_CP040688:1009670-1010104 [Mycobacterium tuberculosis complex sp. N0052]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T126515 NZ_CP040688:1009670-1010104 [Mycobacterium tuberculosis complex sp. N0052]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT126515 WP_003898641.1 NZ_CP040688:1009485-1009664 [Mycobacterium tuberculosis complex sp. N0052]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT126515 NZ_CP040688:1009485-1009664 [Mycobacterium tuberculosis complex sp. N0052]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5