Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 712358..713007 Replicon chromosome
Accession NZ_CP040688
Organism Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag FF951_RS03250 Protein ID WP_003403236.1
Coordinates 712612..713007 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag FF951_RS03245 Protein ID WP_003403235.1
Coordinates 712358..712612 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF951_RS03220 707485..707568 + 84 Protein_638 galactose-1-phosphate uridylyltransferase -
FF951_RS03225 707587..708668 + 1082 Protein_639 galactose-1-phosphate uridylyltransferase -
FF951_RS03230 (FF951_03300) 708665..709756 + 1092 WP_003900977.1 galactokinase -
FF951_RS03235 (FF951_03310) 710150..711214 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
FF951_RS03240 (FF951_03315) 711318..712265 + 948 WP_016810166.1 DUF732 domain-containing protein -
FF951_RS03245 (FF951_03320) 712358..712612 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
FF951_RS03250 (FF951_03325) 712612..713007 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
FF951_RS03255 (FF951_03330) 713101..713841 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
FF951_RS03260 (FF951_03335) 713973..714233 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF951_RS03265 (FF951_03340) 714230..714637 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
FF951_RS03270 (FF951_03345) 714709..715860 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
FF951_RS03275 (FF951_03350) 715953..717680 - 1728 WP_003901883.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T126508 WP_003403236.1 NZ_CP040688:712612-713007 [Mycobacterium tuberculosis complex sp. N0052]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T126508 NZ_CP040688:712612-713007 [Mycobacterium tuberculosis complex sp. N0052]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT126508 WP_003403235.1 NZ_CP040688:712358-712612 [Mycobacterium tuberculosis complex sp. N0052]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT126508 NZ_CP040688:712358-712612 [Mycobacterium tuberculosis complex sp. N0052]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References