Detailed information of TA system TA126508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 712358..713007
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: FF951_RS03250
• Protein ID: WP_003403236.1
• Coordinates: 712612..713007 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: FF951_RS03245
• Protein ID: WP_003403235.1
• Coordinates: 712358..712612 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS03220	707485..707568	+	84	Protein_638	galactose-1-phosphate uridylyltransferase	-
FF951_RS03225	707587..708668	+	1082	Protein_639	galactose-1-phosphate uridylyltransferase	-
FF951_RS03230 (FF951_03300)	708665..709756	+	1092	WP_003900977.1	galactokinase	-
FF951_RS03235 (FF951_03310)	710150..711214	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
FF951_RS03240 (FF951_03315)	711318..712265	+	948	WP_016810166.1	DUF732 domain-containing protein	-
FF951_RS03245 (FF951_03320)	712358..712612	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
FF951_RS03250 (FF951_03325)	712612..713007	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
FF951_RS03255 (FF951_03330)	713101..713841	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
FF951_RS03260 (FF951_03335)	713973..714233	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FF951_RS03265 (FF951_03340)	714230..714637	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
FF951_RS03270 (FF951_03345)	714709..715860	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
FF951_RS03275 (FF951_03350)	715953..717680	-	1728	WP_003901883.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T126508 WP_003403236.1 NZ_CP040688:712612-713007 [Mycobacterium tuberculosis complex sp. N0052]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T126508 NZ_CP040688:712612-713007 [Mycobacterium tuberculosis complex sp. N0052]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT126508 WP_003403235.1 NZ_CP040688:712358-712612 [Mycobacterium tuberculosis complex sp. N0052]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT126508 NZ_CP040688:712358-712612 [Mycobacterium tuberculosis complex sp. N0052]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2