Detailed information of TA system TA126504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 690788..691434
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: FF951_RS03095
• Protein ID: WP_003403122.1
• Coordinates: 690788..691180 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: FF951_RS03100
• Protein ID: WP_003403125.1
• Coordinates: 691177..691434 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS03080 (FF951_03135)	686450..687976	+	1527	WP_003403112.1	virulence factor Mce family protein	-
FF951_RS03085 (FF951_03140)	688039..689181	+	1143	WP_003911253.1	virulence factor Mce family protein	-
FF951_RS03090 (FF951_03145)	689186..690736	+	1551	WP_003403119.1	MlaD family protein	-
FF951_RS03095 (FF951_03150)	690788..691180	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
FF951_RS03100 (FF951_03155)	691177..691434	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
FF951_RS03105 (FF951_03160)	691617..692852	-	1236	WP_003403128.1	ATP-binding protein	-
FF951_RS03110 (FF951_03165)	693103..693516	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
FF951_RS03115 (FF951_03170)	693513..693749	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	-
FF951_RS03120 (FF951_03175)	693853..694359	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FF951_RS03125 (FF951_03180)	694473..695003	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FF951_RS03130 (FF951_03185)	694987..695682	-	696	WP_003918099.1	two-component system response regulator TcrA	-
FF951_RS03135 (FF951_03190)	695805..696116	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T126504 WP_003403122.1 NZ_CP040688:c691180-690788 [Mycobacterium tuberculosis complex sp. N0052]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T126504 NZ_CP040688:c691180-690788 [Mycobacterium tuberculosis complex sp. N0052]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT126504 WP_003403125.1 NZ_CP040688:c691434-691177 [Mycobacterium tuberculosis complex sp. N0052]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT126504 NZ_CP040688:c691434-691177 [Mycobacterium tuberculosis complex sp. N0052]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86