Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3233410..3234024 Replicon chromosome
Accession NZ_CP040684
Organism Pseudomonas aeruginosa strain C79

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag FF962_RS15580 Protein ID WP_071534354.1
Coordinates 3233842..3234024 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag FF962_RS15575 Protein ID WP_012077229.1
Coordinates 3233410..3233814 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF962_RS15550 3229550..3230260 + 711 WP_012074129.1 hypothetical protein -
FF962_RS15555 3230257..3231177 + 921 WP_012074128.1 hypothetical protein -
FF962_RS15560 3231174..3231713 + 540 WP_012074127.1 hypothetical protein -
FF962_RS15565 3231713..3232411 + 699 WP_033896049.1 hypothetical protein -
FF962_RS15570 3232396..3233367 + 972 WP_012077228.1 hypothetical protein -
FF962_RS15575 3233410..3233814 - 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FF962_RS15580 3233842..3234024 - 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
FF962_RS15585 3234441..3234998 + 558 Protein_2993 ABC transporter ATP-binding protein -
FF962_RS15590 3235003..3235896 + 894 WP_003092241.1 ABC transporter permease -
FF962_RS15595 3235889..3236659 + 771 WP_003092244.1 ABC transporter permease -
FF962_RS15600 3236748..3237812 + 1065 WP_017002184.1 spermidine/putrescine ABC transporter substrate-binding protein -
FF962_RS15605 3237988..3238398 + 411 WP_003092248.1 quorum-sensing-regulated virulence factor family protein -
FF962_RS15610 3238417..3238638 + 222 WP_003092249.1 DUF3820 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3201585..3234024 32439


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T126490 WP_071534354.1 NZ_CP040684:c3234024-3233842 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T126490 NZ_CP040684:c3234024-3233842 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT126490 WP_012077229.1 NZ_CP040684:c3233814-3233410 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT126490 NZ_CP040684:c3233814-3233410 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References