Detailed information of TA system TA126484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3754726..3755342
• Replicon: chromosome
• Accession: NZ_CP040677
• Organism: Kosakonia sacchari strain DSM 107661

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1G4ZCB1
• Locus tag: FGE25_RS17940
• Protein ID: WP_017459990.1
• Coordinates: 3755124..3755342 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: FGE25_RS17935
• Protein ID: WP_045509164.1
• Coordinates: 3754726..3755100 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGE25_RS17925	3749832..3751025	+	1194	WP_045509162.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
FGE25_RS17930	3751048..3754197	+	3150	WP_020456808.1	multidrug efflux RND transporter permease subunit	-
FGE25_RS17935	3754726..3755100	+	375	WP_045509164.1	Hha toxicity modulator TomB	Antitoxin
FGE25_RS17940	3755124..3755342	+	219	WP_017459990.1	hemolysin expression modulator Hha	Toxin
FGE25_RS17945	3755419..3755985	+	567	WP_020456806.1	maltose O-acetyltransferase	-
FGE25_RS17950	3756086..3756565	+	480	WP_160271062.1	YlaC family protein	-
FGE25_RS17955	3756531..3757979	-	1449	WP_160271063.1	PLP-dependent aminotransferase family protein	-
FGE25_RS17960	3758080..3758781	+	702	WP_160271064.1	GNAT family N-acetyltransferase	-
FGE25_RS17965	3758778..3758918	-	141	WP_025263710.1	type B 50S ribosomal protein L36	-
FGE25_RS17970	3758922..3759182	-	261	WP_160271532.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8651.08 Da
• Isoelectric Point: 7.9893

>T126484 WP_017459990.1 NZ_CP040677:3755124-3755342 [Kosakonia sacchari]
MSEKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T126484 NZ_CP040677:3755124-3755342 [Kosakonia sacchari]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGACGCTGTCAGACAATTGATACCCTTGAACGTGT
AATTGAAAAAAATAAATATGAACTTCCCGACGAGGAACTCGCGGTGTTTTATTCAGCAGCGGATCATCGTCTGGCAGAAC
TGACCATGAATAAGCTTTATGACAAAATCCCCTCCTCCGTGTGGAAGTTTGTGCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14565.38 Da
• Isoelectric Point: 5.8346

>AT126484 WP_045509164.1 NZ_CP040677:3754726-3755100 [Kosakonia sacchari]
MDEYSPKRHDIAQLKFLCETLYHDCLVNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTHDLQKWRKTGNKLFRSFVNVSKANPVSHSC

Nucleotide

• Length: 375 bp

>AT126484 NZ_CP040677:3754726-3755100 [Kosakonia sacchari]
ATGGACGAATACTCGCCGAAAAGGCATGATATTGCGCAGCTCAAATTTCTCTGCGAAACGTTGTACCATGATTGCCTCGT
AAATCTTGAAGAGAGCCATCATGGGTGGGTTAACGATCCTACTTCAGCAATCAATTTGCAGTTAAACGAGCTGATAGAAC
ACATAGCCACTTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAACAGATAGACGAATACCTC
GACGATACGTTTATGTTGTTCAGTAGCTATGGCATAAATACACACGACTTACAGAAATGGCGTAAAACAGGTAACAAATT
GTTCCGCAGTTTCGTCAATGTAAGCAAGGCTAATCCTGTTAGCCACTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1G4ZCB1