Detailed information of TA system TA126470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2212766..2213683
• Replicon: chromosome
• Accession: NZ_CP040672
• Organism: Bacillus amyloliquefaciens strain X030

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: FGE23_RS11465
• Protein ID: WP_057079950.1
• Coordinates: 2212937..2213683 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: FGE23_RS11460
• Protein ID: WP_003154807.1
• Coordinates: 2212766..2212936 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGE23_RS11425	2207999..2209621	+	1623	WP_146276585.1	pyocin knob domain-containing protein	-
FGE23_RS11430	2209634..2210005	+	372	WP_070081681.1	hypothetical protein	-
FGE23_RS11435	2210010..2210207	+	198	WP_003154819.1	XkdX family protein	-
FGE23_RS11440	2210264..2211025	+	762	WP_146276587.1	phage portal protein	-
FGE23_RS11445	2211077..2211340	+	264	WP_146276589.1	hemolysin XhlA family protein	-
FGE23_RS11450	2211354..2211617	+	264	WP_003154813.1	phage holin	-
FGE23_RS11455	2211631..2212509	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
FGE23_RS11460	2212766..2212936	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
FGE23_RS11465	2212937..2213683	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FGE23_RS11470	2213788..2214786	-	999	WP_003154805.1	inorganic phosphate transporter	-
FGE23_RS11475	2214799..2215416	-	618	WP_003154804.1	DUF47 domain-containing protein	-
FGE23_RS11480	2215702..2217018	-	1317	WP_003154801.1	amino acid permease	-
FGE23_RS11485	2217341..2218291	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2180209..2218291	38082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T126470 WP_057079950.1 NZ_CP040672:c2213683-2212937 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T126470 NZ_CP040672:c2213683-2212937 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT126470 WP_003154807.1 NZ_CP040672:c2212936-2212766 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT126470 NZ_CP040672:c2212936-2212766 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14