Detailed information of TA system TA126469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1477785..1478422
• Replicon: chromosome
• Accession: NZ_CP040672
• Organism: Bacillus amyloliquefaciens strain X030

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FGE23_RS07520
• Protein ID: WP_003156187.1
• Coordinates: 1478072..1478422 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: FGE23_RS07515
• Protein ID: WP_003156188.1
• Coordinates: 1477785..1478066 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGE23_RS07495	1474150..1474749	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
FGE23_RS07500	1474842..1475207	+	366	WP_014304402.1	holo-ACP synthase	-
FGE23_RS07505	1475372..1476379	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
FGE23_RS07510	1476496..1477665	+	1170	WP_146276152.1	alanine racemase	-
FGE23_RS07515	1477785..1478066	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FGE23_RS07520	1478072..1478422	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FGE23_RS07525	1478540..1479361	+	822	WP_003156182.1	STAS domain-containing protein	-
FGE23_RS07530	1479366..1479731	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
FGE23_RS07535	1479734..1480135	+	402	WP_003156178.1	anti-sigma regulatory factor	-
FGE23_RS07540	1480147..1481154	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
FGE23_RS07545	1481218..1481547	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
FGE23_RS07550	1481544..1482026	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
FGE23_RS07555	1481992..1482780	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
FGE23_RS07560	1482780..1483382	+	603	WP_146276154.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T126469 WP_003156187.1 NZ_CP040672:1478072-1478422 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T126469 NZ_CP040672:1478072-1478422 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT126469 WP_003156188.1 NZ_CP040672:1477785-1478066 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT126469 NZ_CP040672:1477785-1478066 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5