Detailed information of TA system TA126457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2922036..2922654
• Replicon: chromosome
• Accession: NZ_CP040667
• Organism: Escherichia coli strain S17-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: FGH86_RS14190
• Protein ID: WP_001290581.1
• Coordinates: 2922436..2922654 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: FGH86_RS14185
• Protein ID: WP_000344800.1
• Coordinates: 2922036..2922410 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGH86_RS14175	2917125..2918318	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
FGH86_RS14180	2918341..2921490	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
FGH86_RS14185	2922036..2922410	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
FGH86_RS14190	2922436..2922654	+	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
FGH86_RS14195	2922826..2923377	+	552	WP_000102564.1	maltose O-acetyltransferase	-
FGH86_RS14200	2923493..2923963	+	471	WP_000136192.1	YlaC family protein	-
FGH86_RS14205	2924127..2925677	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
FGH86_RS14210	2925719..2926072	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
FGH86_RS14220	2926451..2926762	+	312	WP_000409911.1	MGMT family protein	-
FGH86_RS14225	2926793..2927365	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	formA / ant(3'')-Ia / dfrA1	acrA / acrB	2888890..3162826	273936

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T126457 WP_001290581.1 NZ_CP040667:2922436-2922654 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T126457 NZ_CP040667:2922436-2922654 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT126457 WP_000344800.1 NZ_CP040667:2922036-2922410 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT126457 NZ_CP040667:2922036-2922410 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5