Detailed information of TA system TA126456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1905808..1906446
• Replicon: chromosome
• Accession: NZ_CP040667
• Organism: Escherichia coli strain S17-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FGH86_RS09235
• Protein ID: WP_000813794.1
• Coordinates: 1906270..1906446 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FGH86_RS09230
• Protein ID: WP_001270286.1
• Coordinates: 1905808..1906224 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGH86_RS09210	1900960..1901901	-	942	WP_001251304.1	ABC transporter permease	-
FGH86_RS09215	1901902..1902915	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FGH86_RS09220	1902933..1904078	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FGH86_RS09225	1904323..1905729	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
FGH86_RS09230	1905808..1906224	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FGH86_RS09235	1906270..1906446	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FGH86_RS09240	1906668..1906898	+	231	WP_000494244.1	YncJ family protein	-
FGH86_RS09245	1906990..1908951	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FGH86_RS09250	1909024..1909560	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FGH86_RS09255	1909613..1910827	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T126456 WP_000813794.1 NZ_CP040667:c1906446-1906270 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T126456 NZ_CP040667:c1906446-1906270 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT126456 WP_001270286.1 NZ_CP040667:c1906224-1905808 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT126456 NZ_CP040667:c1906224-1905808 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB