Detailed information of TA system TA126445

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1337366..1338197
• Replicon: chromosome
• Accession: NZ_CP040667
• Organism: Escherichia coli strain S17-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FGH86_RS06300
• Protein ID: WP_000854814.1
• Coordinates: 1337366..1337740 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FGH86_RS06305
• Protein ID: WP_001285584.1
• Coordinates: 1337829..1338197 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGH86_RS06260	1332762..1333928	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
FGH86_RS06265	1334047..1334520	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FGH86_RS06270	1334718..1335776	+	1059	WP_001200891.1	FUSC family protein	-
FGH86_RS06275	1335948..1336277	+	330	WP_000450409.1	DUF496 family protein	-
FGH86_RS06280	1336378..1336560	-	183	WP_001016348.1	hypothetical protein	-
FGH86_RS06285	1336632..1336760	+	129	Protein_1228	transposase domain-containing protein	-
FGH86_RS06290	1337049..1337162	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FGH86_RS06295	1337175..1337369	-	195	WP_000988600.1	hypothetical protein	-
FGH86_RS06300	1337366..1337740	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FGH86_RS06305	1337829..1338197	-	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FGH86_RS06310	1338271..1338492	-	222	WP_000692323.1	DUF987 domain-containing protein	-
FGH86_RS06315	1338555..1339001	-	447	WP_000187523.1	RadC family protein	-
FGH86_RS06320	1338998..1340530	-	1533	WP_001350525.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T126445 WP_000854814.1 NZ_CP040667:c1337740-1337366 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T126445 NZ_CP040667:c1337740-1337366 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT126445 WP_001285584.1 NZ_CP040667:c1338197-1337829 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT126445 NZ_CP040667:c1338197-1337829 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6