Detailed information of TA system TA126381

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 263260..263939
• Replicon: chromosome
• Accession: NZ_CP040664
• Organism: Escherichia coli strain KR2009

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FE384_RS01240
• Protein ID: WP_000854672.1
• Coordinates: 263260..263601 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FE384_RS01245
• Protein ID: WP_000070395.1
• Coordinates: 263622..263939 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE384_RS01215	258609..258938	+	330	Protein_238	sigma factor-binding protein Crl	-
FE384_RS01220	258977..260032	-	1056	WP_000749863.1	phosphoporin PhoE	-
FE384_RS01225	260320..261423	+	1104	WP_001285288.1	glutamate 5-kinase	-
FE384_RS01230	261435..262688	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FE384_RS01240	263260..263601	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FE384_RS01245	263622..263939	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FE384_RS01250	263958..264179	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FE384_RS01255	264188..264664	-	477	WP_000811693.1	RadC family protein	-
FE384_RS01260	264680..265138	-	459	WP_000211838.1	antirestriction protein	-
FE384_RS01265	265236..265475	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FE384_RS01270	265552..266019	-	468	WP_001547765.1	hypothetical protein	-
FE384_RS01275	266042..266485	-	444	WP_000824223.1	hypothetical protein	-
FE384_RS01280	266485..266712	-	228	WP_001548158.1	hypothetical protein	-
FE384_RS01285	266708..266899	-	192	Protein_251	DeoR family transcriptional regulator	-
FE384_RS01290	267116..267937	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FE384_RS01295	268029..268892	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T126381 WP_000854672.1 NZ_CP040664:c263601-263260 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T126381 NZ_CP040664:c263601-263260 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT126381 WP_000070395.1 NZ_CP040664:c263939-263622 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT126381 NZ_CP040664:c263939-263622 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684