Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5154928..5155553 Replicon chromosome
Accession NZ_CP040660
Organism Klebsiella pneumoniae strain 2019011308

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FE373_RS24835 Protein ID WP_002882817.1
Coordinates 5154928..5155311 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FE373_RS24840 Protein ID WP_004150355.1
Coordinates 5155311..5155553 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE373_RS24820 (5152294) 5152294..5153196 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FE373_RS24825 (5153193) 5153193..5153828 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FE373_RS24830 (5153825) 5153825..5154754 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FE373_RS24835 (5154928) 5154928..5155311 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FE373_RS24840 (5155311) 5155311..5155553 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FE373_RS24845 (5155758) 5155758..5156675 + 918 WP_004181612.1 alpha/beta hydrolase -
FE373_RS24850 (5156689) 5156689..5157630 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FE373_RS24855 (5157675) 5157675..5158112 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FE373_RS24860 (5158109) 5158109..5158969 - 861 WP_004146232.1 virulence factor BrkB family protein -
FE373_RS24865 (5158963) 5158963..5159562 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T126333 WP_002882817.1 NZ_CP040660:c5155311-5154928 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T126333 NZ_CP040660:c5155311-5154928 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT126333 WP_004150355.1 NZ_CP040660:c5155553-5155311 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT126333 NZ_CP040660:c5155553-5155311 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References