Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 50279..51015 Replicon plasmid p.SXKP01030601
Accession NZ_CP040658
Organism Klebsiella pneumoniae strain 2019010306

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag E3148_RS26125 Protein ID WP_003026803.1
Coordinates 50533..51015 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag E3148_RS26120 Protein ID WP_003026799.1
Coordinates 50279..50545 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3148_RS26075 (46341) 46341..46703 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
E3148_RS26080 (46753) 46753..47103 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
E3148_RS26085 (47461) 47461..47730 + 270 WP_004152102.1 hypothetical protein -
E3148_RS26090 (47718) 47718..48293 + 576 WP_004152103.1 hypothetical protein -
E3148_RS26095 (48324) 48324..48818 + 495 WP_004152104.1 DNA-binding protein -
E3148_RS26100 (48862) 48862..49230 + 369 WP_004152105.1 hypothetical protein -
E3148_RS26105 (49264) 49264..49467 + 204 WP_004152106.1 HHA domain-containing protein -
E3148_RS26110 (49516) 49516..49773 + 258 WP_004152107.1 hypothetical protein -
E3148_RS26115 (49849) 49849..50103 + 255 WP_004152108.1 hypothetical protein -
E3148_RS26120 (50279) 50279..50545 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
E3148_RS26125 (50533) 50533..51015 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
E3148_RS26130 (51227) 51227..52573 + 1347 WP_020314316.1 ISNCY family transposase -
E3148_RS26135 (52734) 52734..52865 + 132 WP_004218042.1 hypothetical protein -
E3148_RS26140 (53074) 53074..54239 - 1166 Protein_56 IS3 family transposase -
E3148_RS26145 (54416) 54416..55378 - 963 WP_004152113.1 zinc metalloprotease HtpX -
E3148_RS26150 (55365) 55365..55853 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139734 139734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T126314 WP_003026803.1 NZ_CP040658:50533-51015 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T126314 NZ_CP040658:50533-51015 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT126314 WP_003026799.1 NZ_CP040658:50279-50545 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT126314 NZ_CP040658:50279-50545 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References