Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5153736..5154361 Replicon chromosome
Accession NZ_CP040657
Organism Klebsiella pneumoniae strain 2019010306

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag E3148_RS24830 Protein ID WP_002882817.1
Coordinates 5153736..5154119 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag E3148_RS24835 Protein ID WP_004150355.1
Coordinates 5154119..5154361 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3148_RS24815 (5151102) 5151102..5152004 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
E3148_RS24820 (5152001) 5152001..5152636 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
E3148_RS24825 (5152633) 5152633..5153562 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
E3148_RS24830 (5153736) 5153736..5154119 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
E3148_RS24835 (5154119) 5154119..5154361 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
E3148_RS24840 (5154566) 5154566..5155483 + 918 WP_004181612.1 alpha/beta hydrolase -
E3148_RS24845 (5155497) 5155497..5156438 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
E3148_RS24850 (5156483) 5156483..5156920 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
E3148_RS24855 (5156917) 5156917..5157777 - 861 WP_004146232.1 virulence factor BrkB family protein -
E3148_RS24860 (5157771) 5157771..5158370 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T126313 WP_002882817.1 NZ_CP040657:c5154119-5153736 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T126313 NZ_CP040657:c5154119-5153736 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT126313 WP_004150355.1 NZ_CP040657:c5154361-5154119 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT126313 NZ_CP040657:c5154361-5154119 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References