Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4821737..4822253 Replicon chromosome
Accession NZ_CP040657
Organism Klebsiella pneumoniae strain 2019010306

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag E3148_RS23255 Protein ID WP_004178374.1
Coordinates 4821737..4822021 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag E3148_RS23260 Protein ID WP_002886901.1
Coordinates 4822011..4822253 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3148_RS23230 (4817221) 4817221..4817484 - 264 WP_020324507.1 PTS system, lactose/cellobiose-specific IIB subunit -
E3148_RS23235 (4817614) 4817614..4817787 + 174 WP_032408826.1 hypothetical protein -
E3148_RS23240 (4817790) 4817790..4818533 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
E3148_RS23245 (4818890) 4818890..4821028 + 2139 WP_004186701.1 anaerobic ribonucleoside-triphosphate reductase -
E3148_RS23250 (4821269) 4821269..4821733 + 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
E3148_RS23255 (4821737) 4821737..4822021 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3148_RS23260 (4822011) 4822011..4822253 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
E3148_RS23265 (4822331) 4822331..4824241 - 1911 WP_004178373.1 PRD domain-containing protein -
E3148_RS23270 (4824264) 4824264..4825418 - 1155 WP_004178372.1 lactonase family protein -
E3148_RS23275 (4825485) 4825485..4826225 - 741 WP_002886899.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T126311 WP_004178374.1 NZ_CP040657:c4822021-4821737 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T126311 NZ_CP040657:c4822021-4821737 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT126311 WP_002886901.1 NZ_CP040657:c4822253-4822011 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT126311 NZ_CP040657:c4822253-4822011 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References