Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4076860..4077479 Replicon chromosome
Accession NZ_CP040657
Organism Klebsiella pneumoniae strain 2019010306

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag E3148_RS19800 Protein ID WP_002892050.1
Coordinates 4077261..4077479 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag E3148_RS19795 Protein ID WP_002892066.1
Coordinates 4076860..4077234 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3148_RS19785 (4072012) 4072012..4073205 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E3148_RS19790 (4073228) 4073228..4076374 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
E3148_RS19795 (4076860) 4076860..4077234 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
E3148_RS19800 (4077261) 4077261..4077479 + 219 WP_002892050.1 HHA domain-containing protein Toxin
E3148_RS19805 (4077638) 4077638..4078204 + 567 WP_002892030.1 maltose O-acetyltransferase -
E3148_RS19810 (4078176) 4078176..4078316 - 141 WP_004147370.1 hypothetical protein -
E3148_RS19815 (4078337) 4078337..4078807 + 471 WP_002892026.1 YlaC family protein -
E3148_RS19820 (4078782) 4078782..4080233 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
E3148_RS19825 (4080334) 4080334..4081032 + 699 WP_002892021.1 GNAT family protein -
E3148_RS19830 (4081029) 4081029..4081169 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
E3148_RS19835 (4081169) 4081169..4081432 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T126309 WP_002892050.1 NZ_CP040657:4077261-4077479 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T126309 NZ_CP040657:4077261-4077479 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT126309 WP_002892066.1 NZ_CP040657:4076860-4077234 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT126309 NZ_CP040657:4076860-4077234 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References