Detailed information of TA system TA126297

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 700961..701802
• Replicon: chromosome
• Accession: NZ_CP040657
• Organism: Klebsiella pneumoniae strain 2019010306

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: E3148_RS03470
• Protein ID: WP_000854822.1
• Coordinates: 701419..701802 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0CWL2
• Locus tag: E3148_RS03465
• Protein ID: WP_053271974.1
• Coordinates: 700961..701329 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3148_RS03445 (698706)	698706..699524	+	819	WP_001234702.1	DUF932 domain-containing protein	-
E3148_RS03450 (699615)	699615..700100	+	486	WP_000214317.1	antirestriction protein	-
E3148_RS03455 (700115)	700115..700591	+	477	WP_001186747.1	RadC family protein	-
E3148_RS03460 (700660)	700660..700881	+	222	WP_000692312.1	DUF987 domain-containing protein	-
E3148_RS03465 (700961)	700961..701329	+	369	WP_053271974.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E3148_RS03470 (701419)	701419..701802	+	384	WP_000854822.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
E3148_RS03475 (701793)	701793..702281	+	489	WP_001177592.1	DUF5983 family protein	-
E3148_RS03480 (702248)	702248..702490	+	243	WP_166635458.1	DUF957 domain-containing protein	-
E3148_RS03485 (702587)	702587..703432	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
E3148_RS03495 (703731)	703731..704237	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
E3148_RS03500 (704337)	704337..706178	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(3)-IId	fimD / fimF / fimG	674535..708142	33607

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T126297 WP_000854822.1 NZ_CP040657:701419-701802 [Klebsiella pneumoniae]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T126297 NZ_CP040657:701419-701802 [Klebsiella pneumoniae]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13679.41 Da
• Isoelectric Point: 6.4758

>AT126297 WP_053271974.1 NZ_CP040657:700961-701329 [Klebsiella pneumoniae]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT126297 NZ_CP040657:700961-701329 [Klebsiella pneumoniae]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1

Antitoxin

• AlphaFold DB: A0A1M0CWL2