Detailed information of TA system TA126236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 623083..623708
• Replicon: chromosome
• Accession: NZ_CP040640
• Organism: Agrobacterium sp. T29

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FGF54_RS03215
• Protein ID: WP_125144642.1
• Coordinates: 623349..623708 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A420FBH4
• Locus tag: FGF54_RS03210
• Protein ID: WP_059753836.1
• Coordinates: 623083..623349 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGF54_RS03185	618109..618933	+	825	WP_020811374.1	class D beta-lactamase	-
FGF54_RS03190	618975..619583	+	609	WP_142778904.1	class I SAM-dependent methyltransferase	-
FGF54_RS03195	619759..622341	+	2583	WP_168207991.1	heavy metal translocating P-type ATPase	-
FGF54_RS03200	622338..622760	+	423	WP_142778905.1	Cu(I)-responsive transcriptional regulator	-
FGF54_RS03205	622814..623014	+	201	WP_080817386.1	heavy-metal-associated domain-containing protein	-
FGF54_RS03210	623083..623349	+	267	WP_059753836.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FGF54_RS03215	623349..623708	+	360	WP_125144642.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FGF54_RS03220	623729..623977	-	249	WP_142778906.1	hypothetical protein	-
FGF54_RS03225	624157..625566	+	1410	WP_210247430.1	MFS transporter	-
FGF54_RS03230	625557..625730	-	174	WP_078051557.1	hypothetical protein	-
FGF54_RS03235	625799..627514	-	1716	WP_142778907.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-115)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12924.08 Da
• Isoelectric Point: 8.2824

>T126236 WP_125144642.1 NZ_CP040640:623349-623708 [Agrobacterium sp. T29]
MVRSQVPKRGDVYLVDLNPVIGSEIRDEHRCIVITPREINAVGLCLVVPVTTGGVFTRRAGLAVNISGHRTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPV

Nucleotide

• Length: 360 bp

>T126236 NZ_CP040640:623349-623708 [Agrobacterium sp. T29]
ATGGTCCGCAGCCAGGTTCCAAAACGTGGCGACGTTTATCTGGTCGACCTCAATCCTGTCATCGGCAGCGAAATCAGGGA
CGAGCATCGTTGTATCGTCATCACGCCCAGAGAAATCAACGCGGTTGGACTGTGCCTTGTCGTTCCCGTGACGACGGGTG
GAGTATTCACACGCAGGGCAGGGCTTGCCGTGAACATATCGGGCCACAGGACAACGGGTGTCGCGCTATGCAATCAGGTG
AGAAGCATGGACATTGTTGCCCGTGTCGCCCAGAAAAAAGCGAAATATATCGAAACCCTTGACGATGCGACGATCGACGA
AATCGCCGGTCGTGTCATCAGCATGATCGATCCCGTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9424.90 Da
• Isoelectric Point: 4.5983

>AT126236 WP_059753836.1 NZ_CP040640:623083-623349 [Agrobacterium sp. T29]
MTVTTRIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRQEKKRFTLAELLEGADEVAALNASAREWDEA
PPVGKEAL

Nucleotide

• Length: 267 bp

>AT126236 NZ_CP040640:623083-623349 [Agrobacterium sp. T29]
ATGACCGTAACGACGAGAATAAGACGTCAGGGCGGTGCGGCGGTGATGACCATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAACAACTGACACTTGAGGTCGACAACGGCGCGCTGGTCGCCAGCCCGGTTCGGCAGGAAAAGAAGC
GCTTCACGCTTGCAGAACTGCTGGAGGGTGCGGATGAAGTCGCCGCCCTCAATGCCAGCGCGCGGGAATGGGACGAAGCG
CCGCCAGTTGGTAAAGAAGCCCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A420FBH4