Detailed information of TA system TA126231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3562370..3562931
• Replicon: chromosome
• Accession: NZ_CP040634
• Organism: Acidipropionibacterium acidipropionici strain FAM 19036

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FEZ30_RS16350
• Protein ID: WP_138575163.1
• Coordinates: 3562584..3562931 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K7S105
• Locus tag: FEZ30_RS16345
• Protein ID: WP_015071955.1
• Coordinates: 3562370..3562597 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEZ30_RS16330	3559380..3559784	+	405	WP_015071959.1	nitrite reductase small subunit NirD	-
FEZ30_RS16335	3559894..3562044	-	2151	WP_138575161.1	NAD(+) synthase	-
FEZ30_RS16340	3562022..3562213	-	192	WP_036936157.1	hypothetical protein	-
FEZ30_RS16345	3562370..3562597	+	228	WP_015071955.1	ribbon-helix-helix domain-containing protein	Antitoxin
FEZ30_RS16350	3562584..3562931	+	348	WP_138575163.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FEZ30_RS16355	3563087..3564046	-	960	WP_138575165.1	carbohydrate kinase	-
FEZ30_RS16360	3564213..3565760	+	1548	WP_138575167.1	glycoside hydrolase family 32 protein	-
FEZ30_RS16365	3566290..3566667	+	378	WP_138575520.1	L,D-transpeptidase	-
FEZ30_RS16370	3566772..3567797	-	1026	WP_138575169.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-35)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12419.41 Da
• Isoelectric Point: 11.5180

>T126231 WP_138575163.1 NZ_CP040634:3562584-3562931 [Acidipropionibacterium acidipropionici]
MIRGEVRLVDLDPARPGEANKRRPAVLVSNDTANRTAARLGRGVVTVVPVTSNVTRVFPFQVLLQADETGLRLDSKAQAE
QVRSVSVARIGPVLGMVPADLMSRIDDALRLHLGL

Nucleotide

• Length: 348 bp

>T126231 NZ_CP040634:3562584-3562931 [Acidipropionibacterium acidipropionici]
ATGATCCGCGGTGAGGTCCGGCTGGTCGATCTGGATCCGGCGCGCCCCGGCGAGGCGAACAAGCGACGTCCCGCCGTCCT
CGTGAGCAATGACACGGCGAACCGGACGGCCGCACGGCTGGGGCGGGGAGTGGTGACGGTGGTGCCGGTCACCAGCAACG
TCACCCGGGTCTTCCCCTTCCAGGTCCTGCTTCAGGCGGACGAGACGGGGCTGCGGCTCGATTCCAAGGCCCAGGCCGAG
CAGGTGCGCTCGGTGTCTGTGGCGCGGATCGGGCCGGTGCTCGGCATGGTGCCGGCTGATCTCATGAGCCGCATCGATGA
CGCGCTGAGACTCCACCTGGGGCTGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8161.01 Da
• Isoelectric Point: 4.0941

>AT126231 WP_015071955.1 NZ_CP040634:3562370-3562597 [Acidipropionibacterium acidipropionici]
MKLSVSLSKEQVETIDRYIRATGMRSRSAVIQKAIGMLGAPELEGDYAAAWDEWESGDAEAWESTAADGLGDDPR

Nucleotide

• Length: 228 bp

>AT126231 NZ_CP040634:3562370-3562597 [Acidipropionibacterium acidipropionici]
ATGAAACTCAGTGTCAGCCTGTCCAAGGAGCAGGTGGAGACCATCGACCGGTACATCCGTGCCACCGGGATGAGGTCGAG
GTCTGCGGTGATCCAGAAGGCGATCGGCATGCTCGGCGCCCCGGAGCTCGAGGGGGACTACGCCGCCGCCTGGGACGAGT
GGGAGTCCGGGGACGCCGAGGCCTGGGAGTCGACGGCCGCCGATGGGCTCGGCGATGATCCGCGGTGA

Structures

Antitoxin

• AlphaFold DB: K7S105