Detailed information of TA system TA126181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2231130..2231659
• Replicon: chromosome
• Accession: NZ_CP040622
• Organism: Staphylococcus aureus strain JKD6004

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FF958_RS11490
• Protein ID: WP_000621175.1
• Coordinates: 2231130..2231492 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FF958_RS11495
• Protein ID: WP_000948331.1
• Coordinates: 2231489..2231659 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF958_RS11470	2228109..2228879	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
FF958_RS11475	2228854..2229333	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FF958_RS11480	2229335..2229661	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FF958_RS11485	2229780..2230781	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FF958_RS11490	2231130..2231492	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FF958_RS11495	2231489..2231659	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FF958_RS11500	2231744..2232892	-	1149	WP_001281145.1	alanine racemase	-
FF958_RS11505	2232958..2233317	-	360	WP_000581200.1	holo-ACP synthase	-
FF958_RS11510	2233321..2233812	-	492	WP_001205910.1	PH domain-containing protein	-
FF958_RS11515	2233799..2235382	-	1584	WP_001294626.1	PH domain-containing protein	-
FF958_RS11520	2235375..2235854	-	480	WP_001287088.1	hypothetical protein	-
FF958_RS11525	2236062..2236622	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T126181 WP_000621175.1 NZ_CP040622:c2231492-2231130 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T126181 NZ_CP040622:c2231492-2231130 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT126181 WP_000948331.1 NZ_CP040622:c2231659-2231489 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT126181 NZ_CP040622:c2231659-2231489 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1