Detailed information of TA system TA126132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 84210..85013
• Replicon: plasmid pKpvST15_NDM-1
• Accession: NZ_CP040598
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: W8E895
• Locus tag: FFY44_RS29000
• Protein ID: WP_016479963.1
• Coordinates: 84483..85013 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: FFY44_RS28995
• Protein ID: WP_022652312.1
• Coordinates: 84210..84479 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFY44_RS28980	80696..80848	-	153	Protein_91	IS5/IS1182 family transposase	-
FFY44_RS28985	81330..82069	+	740	Protein_92	site-specific integrase	-
FFY44_RS28990	82365..83354	-	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
FFY44_RS28995	84210..84479	+	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
FFY44_RS29000	84483..85013	+	531	WP_016479963.1	GNAT family N-acetyltransferase	Toxin
FFY44_RS29005	85145..86161	+	1017	WP_087728544.1	IS5-like element IS5 family transposase	-
FFY44_RS29010	86308..87057	-	750	Protein_97	IS3 family transposase	-
FFY44_RS29015	87067..87186	-	120	Protein_98	IS30 family transposase	-
FFY44_RS29020	87447..87623	-	177	Protein_99	IS91 family transposase	-
FFY44_RS29025	87812..89452	-	1641	WP_004201176.1	chaperonin GroEL	-
FFY44_RS29030	89508..89798	-	291	WP_004201172.1	co-chaperone GroES	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / rmtC / sul1	htpB	1..106597	106597
	-	inside	IScluster/Tn	-	-	62929..86973	24044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20350.24 Da
• Isoelectric Point: 6.4907

>T126132 WP_016479963.1 NZ_CP040598:84483-85013 [Klebsiella pneumoniae subsp. pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T126132 NZ_CP040598:84483-85013 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT126132 WP_022652312.1 NZ_CP040598:84210-84479 [Klebsiella pneumoniae subsp. pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT126132 NZ_CP040598:84210-84479 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6C0L7Q2

Antitoxin

• AlphaFold DB: A0A2Z3XDS5