Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 247146..247882 Replicon plasmid pKpvST15
Accession NZ_CP040595
Organism Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag FFY44_RS27665 Protein ID WP_004098919.1
Coordinates 247400..247882 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag FFY44_RS27660 Protein ID WP_004213599.1
Coordinates 247146..247412 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFY44_RS27640 244658..245663 + 1006 Protein_272 IS110 family transposase -
FFY44_RS27645 245949..246443 + 495 WP_004213594.1 hypothetical protein -
FFY44_RS27650 246504..246707 + 204 WP_004213596.1 hemolysin expression modulator Hha -
FFY44_RS27655 246721..246951 + 231 WP_004213598.1 hypothetical protein -
FFY44_RS27660 247146..247412 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
FFY44_RS27665 247400..247882 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
FFY44_RS27670 248083..249487 + 1405 Protein_278 ISNCY-like element ISKpn21 family transposase -
FFY44_RS27675 249516..250147 - 632 Protein_279 hypothetical protein -
FFY44_RS27680 250497..251534 - 1038 WP_138663956.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-6 / aac(6')-Ib / qacE / sul1 / rmtC iucA / iucA / iucB / iucC / iucD / iutA / rmpA 1..277162 277162
- inside IScluster/Tn aac(6')-Ib / qacE / sul1 / rmtC - 218691..253012 34321


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T126131 WP_004098919.1 NZ_CP040595:247400-247882 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T126131 NZ_CP040595:247400-247882 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT126131 WP_004213599.1 NZ_CP040595:247146-247412 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT126131 NZ_CP040595:247146-247412 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References