Detailed information of TA system TA126130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 23959..24629
• Replicon: plasmid pKpvST15
• Accession: NZ_CP040595
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: FFY44_RS26295
• Protein ID: WP_004213072.1
• Coordinates: 23959..24402 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: FFY44_RS26300
• Protein ID: WP_004213073.1
• Coordinates: 24399..24629 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFY44_RS26255	19041..19316	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
FFY44_RS26260	19379..19870	+	492	WP_011251327.1	DM13 domain-containing protein	-
FFY44_RS26265	19919..20839	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
FFY44_RS26275	21128..22109	-	982	Protein_25	IS5 family transposase	-
FFY44_RS29700	22178..22816	-	639	WP_032488580.1	RmpA2	-
FFY44_RS26285	23232..23525	+	294	Protein_27	transposase	-
FFY44_RS26290	23529..23945	+	417	Protein_28	restriction endonuclease	-
FFY44_RS26295	23959..24402	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFY44_RS26300	24399..24629	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FFY44_RS26305	25237..26370	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
FFY44_RS26310	26386..26679	+	294	WP_004213076.1	hypothetical protein	-
FFY44_RS26315	26669..26875	-	207	WP_004213077.1	hypothetical protein	-
FFY44_RS26320	27227..27517	+	291	WP_004213078.1	hypothetical protein	-
FFY44_RS26325	27507..28406	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-6 / aac(6')-Ib / qacE / sul1 / rmtC	iucA / iucA / iucB / iucC / iucD / iutA / rmpA	1..277162	277162
	-	flank	IS/Tn	-	-	21241..22026	785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T126130 WP_004213072.1 NZ_CP040595:c24402-23959 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T126130 NZ_CP040595:c24402-23959 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT126130 WP_004213073.1 NZ_CP040595:c24629-24399 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT126130 NZ_CP040595:c24629-24399 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620