Detailed information of TA system TA126129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 6127..6592
• Replicon: plasmid unnamed1
• Accession: NZ_CP040594
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: FFY44_RS25970
• Protein ID: WP_001230707.1
• Coordinates: 6341..6592 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: FFY44_RS25965
• Protein ID: WP_000801440.1
• Coordinates: 6127..6354 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFY44_RS25915	1359..1574	-	216	WP_000760375.1	EexN family lipoprotein	-
FFY44_RS25920	1571..1864	-	294	WP_000134733.1	LuxR family transcriptional regulator	-
FFY44_RS25925	1941..3170	-	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
FFY44_RS25930	3174..3677	-	504	WP_000781813.1	hypothetical protein	-
FFY44_RS25940	4038..4385	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
FFY44_RS25945	4382..4630	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
FFY44_RS25950	4935..5285	-	351	WP_000854259.1	hypothetical protein	-
FFY44_RS25955	5376..5675	-	300	WP_000650309.1	hypothetical protein	-
FFY44_RS25965	6127..6354	-	228	WP_000801440.1	hypothetical protein	Antitoxin
FFY44_RS25970	6341..6592	-	252	WP_001230707.1	hypothetical protein	Toxin
FFY44_RS25975	6589..7131	-	543	WP_001083906.1	hypothetical protein	-
FFY44_RS25980	7236..7751	-	516	WP_001025395.1	J domain-containing protein	-
FFY44_RS25990	9166..10032	+	867	WP_001353743.1	replication initiation protein	-
FFY44_RS25995	10045..10449	+	405	WP_012421203.1	hypothetical protein	-
FFY44_RS26000	10584..11111	+	528	WP_000577043.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31184	31184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T126129 WP_001230707.1 NZ_CP040594:c6592-6341 [Klebsiella pneumoniae subsp. pneumoniae]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T126129 NZ_CP040594:c6592-6341 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT126129 WP_000801440.1 NZ_CP040594:c6354-6127 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT126129 NZ_CP040594:c6354-6127 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9